Two new protocols led by Claudia Cattoglio are now published with detailed step-by-step guidelines.
The first provides a detailed protocol for how to measure the absolute abundance (#proteins per cell) of any Halo-Tagged protein in a couple of afternoons using just Flow Cytometry based on our 2019 paper and can be found here: https://bio-protocol.org/e3527
The second protocol provides detailed step-by-step guidance for to carry out robust co-IP experiments, for example of the same protein with two different epitope tags and can be found here: https://bio-protocol.org/e3526

We are officially a lab. Michele Gabriele has joined as the lab's first post-doc and Viraat Goel and Jin (Harvey) Yang have joined as the first two graduate students from MIT's Biological Engineering program. Science is happening!