The ultra-high resolution Micro-C paper in mouse embryonic stem cells is published in Molecular Cell. Besides capturing Compartments, TADs, Loops, Stripes/Flames, Micro-C is uniquely capable of capturing transcription-associated chromatin interactions such as Enhancer-Promoter (E-P) and Promoter-Promoter (P-P) interactions.
This paper was led by Stanley Tsung-Han Hsieh and please read the full story here. A complementary Micro-C paper back-to-back can be found here.
Firm believers in the importance of collegiality, the Hansen lab decided to inaugurate its first lab outing by trading the lab bench for Boston's Boda Borg. After an evening of jumping around, solving puzzles, and learning from our mistakes, we triumphantly completed 3 rooms and returned home. Who knows where we'll head to next...
Two new protocols led by Claudia Cattoglio are now published with detailed step-by-step guidelines.
The first provides a detailed protocol for how to measure the absolute abundance (#proteins per cell) of any Halo-Tagged protein in a couple of afternoons using just Flow Cytometry based on our 2019 paper and can be found here: https://bio-protocol.org/e3527
The second protocol provides detailed step-by-step guidance for to carry out robust co-IP experiments, for example of the same protein with two different epitope tags and can be found here: https://bio-protocol.org/e3526
Preprint on how an RNA-Binding Region (RBRi) in CTCF regulates clustering and chromatin looping is now published in Molecular Cell