Asmita's protocol is published: A Protocol for Studying Transcription Factor Dynamics Using Fast Single-Particle Tracking and Spot-On Model-Based Analysis
Congratulations to Asmita whose protocol paper is now published in Methods in Molecular Biology.
Asmita wrote this step-by-step protocol to help anyone interested in Single-Particle Tracking (SPT) get started with SPT experiments and how to analyze the data. The focus is on transcription factors in mammalian cells, but we also discuss key considerations to avoid common biases in SPT and how to optimize the experiments in general. Finally, we discuss how to analyze "fastSPT" data with Spot-On.
Read the full protocol here: https://link.springer.com/protocol/10.1007%2F978-1-0716-2140-0_9