We are thrilled to share our preprint on the dynamics of chromatin looping.
Using 3D Super-Resolution Live-Cell Imaging, we visualize the CTCF/cohesin-mediated loops that hold together TADs and loop domains for the first time in living cells. Surprisingly, these loops are both highly dynamic (median lifetime of ~10-30 min) and very rare (~3-6.5%). Instead of a stable loop, our results establish a highly dynamic view of TADs and loops where TADs exists predominantly in a partially extruded conformation. Read more here. Data here. Code here.
This was a team effort and only possible due to the amazing lead from Michele, Hugo, Simon, and Asmita who lead the project and put it all together during a global pandemic!
This was a collaborative project with Leonid Mirny (MIT Physics) and Christoph Zechner (Max Planck, Dresden) and the project began in Berkeley in collaboration with Gina Dailey, Claudia Cattoglio, and TH Stanley Hsieh.