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Paper on absolute quantification of CTCF and cohesin is now published in eLife

6/17/2019

 
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Our preprint reporting the absolute quantification of CTCF (~217k proteins per cell) of cohesin (through Rad21; ~109k proteins per cell) in mouse embryonic stem cells has now been published in eLife.
We use these measurements to estimate constraints on models of 3D genome organization: the time-averaged occupancy of an average CTCF binding site is ~50% and the density of putatively loop extruding cohesins is 5.3 per Mb (if monomeric).
We also present biochemical evidence for cohesin-cohesin interactions and we report a very simple, convenient and easy method for absolute abundance quantification of any Halo-tagged protein of interest.
The full paper is here: https://elifesciences.org/articles/40164
And a back-to-back complementary paper in HeLa cells is here: https://elifesciences.org/articles/46269

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Hansen Lab at MIT, Building 56, 21 Ames St, Cambridge, MA 02142